Signalling

Part:BBa_K750004

Designed by: Sifan Wang,Ruosang Qiu,Jianzhao Chi,Muxin Yu   Group: iGEM12_XMU-China   (2012-09-18)

LuxI expression device activated by arabinose(Regulated by RBS of 0.07 strength)

This part contains pBAD promoter, rbs of 0.07 strength, LuxI producer and double terminator. Once the promoter pBAD is activated by arabinose, this part can produce LuxI protein. The LuxI protein synthesize a small, diffusible acyl-homoserinelactone (AHL) signaling molecule. The AHL accumlates as the cell density increases, and it can bind with the LuxR protein. The LuxR-AHL can activate promoter LuxPR, which we used as the promoter of the GFP expression device(BBa_K750006) in our project.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 795
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
controlK206000
device_typesignalling
proteinsLuxI